CRISPR

3D Printed Chips Empower CRISPR for Rapid On-Site Detection of Foodborne Bacteria

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3D Printed Chips Empower CRISPR for Rapid On-Site Detection of Foodborne Bacteria

Recently, Professor Ding Xiong’s team from Southeast University published significant research findings in the journal Advanced Science, successfully developing a deployable platform for the multiplex detection of foodborne bacteria. This platform ingeniously combines an enhanced CRISPR detection system with 3D printed microfluidic chips, providing a revolutionary solution for rapid food safety testing. The team’s enhanced … Read more

CRISPRa Enhances Neuron Reprogramming Speed and Efficiency

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CRISPRa Enhances Neuron Reprogramming Speed and Efficiency

Written by | Qi Edited by | Yan Utilizing neuronal regeneration to treat neurodegenerative diseases remains a key challenge in the field of regenerative medicine. The direct conversion of local glial cells into neurons has become a viable alternative to replace functional neurons【1,2】. Considering that the increase in reactive oxygen species generation significantly hinders the … Read more

Wide Dynamic Range Multiplex Digital CRISPR Chip for Absolute Quantification of Nucleic Acids

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Wide Dynamic Range Multiplex Digital CRISPR Chip for Absolute Quantification of Nucleic Acids

01Research Overview With the rapid development of molecular diagnostic technologies, digital CRISPR technology has become an important tool for nucleic acid detection due to its single-molecule counting and absolute quantification capabilities. However, traditional digital CRISPR platforms face significant challenges in several aspects. Firstly, most existing platforms adopt equal-volume microcavity designs, which typically cover a dynamic … Read more

RNA-Activated CRISPR/Cas12a Nanorobots Operating in Live Cells

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RNA-Activated CRISPR/Cas12a Nanorobots Operating in Live Cells

Abstract The active clustered regularly interspaced short palindromic repeats (CRISPR/Cas12a) system possesses cis-cleavage (targeting) and trans-cleavage (off-target) activities, which are highly useful for genome engineering and diagnostic applications. Both single-stranded and double-stranded DNA can activate crRNA−Cas12a ribonucleoprotein (RNP), enabling its cis and trans cleavage enzymatic activities. However, whether RNA can activate the CRISPR/Cas12a system and … Read more

One-Pot Diagnostic Methods Based on CRISPR/Cas and Argonaute Nucleases

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One-Pot Diagnostic Methods Based on CRISPR/Cas and Argonaute Nucleases

▲ Click the above blue text to follow CellPress Cell Science ▲ Life Science Life science Coupling CRISPR/Cas and Argonaute (Ago) programmable nucleases with isothermal amplification technologies effectively addresses the limitations of isothermal amplification technologies, such as RPA and LAMP, which suffer from poor specificity and difficulty in multiplex detection, providing new solutions for point-of-care … Read more