Cell biologists observe various gene-determined, active, orderly forms of cell death during development, known as programmed cell death, including apoptosis, autophagy, pyroptosis, and ferroptosis. Ferroptosis is a metabolic form of non-apoptotic cell death characterized by iron-dependent lipid peroxidation, and it has garnered significant attention due to its strong correlation with human diseases such as neurodegenerative … Read more
Subcellular organelles in cells often have intricate three-dimensional structures and biological functions. Observing the three-dimensional distribution and dynamics of organelles using fluorescence microscopy helps understand cellular functions and reveals interactions between organelles. However, fluorescence microscopy is limited by optical diffraction and the numerical aperture (NA) of the objective lens, resulting in blurred low-resolution images. Due … Read more
Cytopathic effects (CPEs) are a hallmark of infection. Due to the phototoxicity of classical optical microscopy, CPEs are difficult to observe. This study reports different patterns of viral infection in live cells using Digital HoloTomographic Microscopy (DHTM). DHTM is label-free and records the phase shift of low-energy light passing through the sample on a transparent … Read more