According to reports from Maims Consulting, recently, Professor Shui Lingling’s team at South China Normal University, led by Associate Researcher Liu Zhenping, constructed an integrated electrochemical aptamer sensor array based on a multi-layer honeycomb-like porous carbonized ZIF67 (h-porous C-ZIF67) for the integrated detection of CK-MB, cTnI, and Mb in plasma. This approach addresses the agglomeration issues of traditional nanomaterials on sensor surfaces and provides a new pathway for precise electrochemical detection interface regulation. The related results were published under the title “Precisely engineered honeycomb-like C-ZIF67 aptasensor array for integrated detection of multiple cardiac biomarkers in AMI diagnosis” in Biosensors and Bioelectronics.Early and accurate diagnosis of acute myocardial infarction (AMI) is crucial for reducing mortality and improving treatment outcomes in cardiovascular diseases (CVDs). The integrated detection of biomarkers such as creatine kinase isoenzyme (CK-MB), cardiac troponin I (cTnI), and myoglobin (Mb) provides a reliable strategy for timely diagnosis and prognostic assessment.This study constructed an integrated electrochemical aptamer sensor array modified with multi-layer honeycomb-like porous carbonized ZIF67 (h-porous C-ZIF67) for the integrated detection of CK-MB, cTnI, and Mb in plasma samples.Uniform and controllable array electrode units were constructed using a laser etching process, combined with particle assembly, precursor infiltration, spin coating, and high-temperature carbonization to in situ prepare the honeycomb porous structure C-ZIF67 modification layer. By anchoring specific aptamer sensitive elements and utilizing horseradish peroxidase (HRP) for signal amplification, the detection sensitivity was enhanced. This aptamer sensor array exhibited a wide linear response range from 1.0 pg/mL to 100.0 ng/mL, with detection limits for CK-MB, cTnI, and Mb as low as 0.38 pg/mL, 0.03 pg/mL, and 0.42 pg/mL, respectively.
Figure 1: Schematic diagram of the construction process of the honeycomb porous structure C-ZIF67 for the multi-target cardiac biomarker detection biosensor array. (A) Layered assembly of PS microspheres, (B) Formation process of the honeycomb multi-layer porous structure, (C) In situ modification and construction of the multi-aptamer sensor array, (D) Integrated detection of multiple biomarkers using the aptamer sensor array.Based on this layered assembly strategy, precise control over the thickness and structure of the h-porous C-ZIF67 modification layer was achieved, effectively addressing the reproducibility issues present in traditional surface modification methods. By combining tunable porous materials, arrayed electrode units, aptamer-specific recognition, and catalytic amplification strategies, this electrode array offers advantages such as shorter detection times, better reproducibility, and strong multi-target detection capabilities compared to traditional immunoassays. It has the potential to serve as a point-of-care testing (POCT) platform for early diagnosis of AMI and is expected to expand into broader clinical application scenarios.
Figure 2: FEI-SEM images. (A) Self-assembled PS microspheres, (B) PS template filled with MOF precursor, (C) Honeycomb porous structure C-ZIF67, (D) Magnified view of the honeycomb porous structure C-ZIF67, (E) XRD patterns of FTO, ZIF67, and honeycomb porous structure C-ZIF67, (F) XPS spectra of ZIF67 and honeycomb porous structure C-ZIF67. High-resolution XPS analysis of honeycomb porous structure C-ZIF67: (G) C 1s, (H) N 1s, (I) Co 2p.Paper link:
https://doi.org/10.1016/j.bios.2025.118167
Further Reading:
“Biosensor Technologies and Market for Point-of-Care Diagnostics – 2022 Edition”
