3D-Printed Biomimetic Vascular Scaffold for Sustained Release of PDGFB-LG4 Fusion Protein Promotes Bone Regeneration
Advanced Science ( IF 14.3 ) Pub Date : 2025-03-27 , DOI: 10.1002/advs.202414362
Jiahua Duan, Gaofei Qian, Huikang Zhang, Feifan Wang, Qiang Tian, Dong Lei, Jianning Zhao
The 3D-printed biomimetic vascular scaffold crosslinked with heparan sulfate is used for the sustained release of PDGFB-LG4 fusion protein to promote bone regeneration.
A central focus of bone tissue engineering is the construction of a vascular system to provide nutrients for cell survival, remove metabolic waste, and accelerate tissue regeneration. Platelet-derived growth factor-BB (PDGFB) has the ability to stimulate angiogenesis and bone regeneration; however, its clinical application is hindered by side effects and low efficacy due to suboptimal delivery systems. In this study, a biomimetic vascular scaffold crosslinked with heparan sulfate (HS) was developed for the sustained delivery of PDGFB-LG4 fusion protein, targeting the regeneration of critical-sized bone defects. The scaffold features a vascular-like layered structure design, incorporating a custom 3D framework, multi-branch microchannels, and permeable porous walls to facilitate mass exchange and cell infiltration. Compared to PDGFB, PDGFB-LG4 exhibited superior osteoinductive and angiogenic activity. In a cranial defect model, the composite scaffold (PCLHS-PDGFB-LG4) significantly enhanced angiogenesis and bone regeneration, demonstrating higher efficacy at lower doses compared to PDGFB. This approach may be applicable to other growth factors and gelatin-based materials, showing broad potential in regenerative medicine.
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