I wrote a Makefile to quickly deploy a small project that automates the integration of RNA sequencing via email. This small project automatically clears all files under the entire project directory approximately every week, so quick automated deployment is quite necessary. The format layout presented may have issues, and directly copying it might cause problems; … Read more
Using trim-galore to remove low-quality reads and adaptersAdapters: An adapter is a known short nucleotide sequence used to link unknown target sequencing fragments.Create a new directory “trim-galore” to store the output results of trim-galore.1. If running a single sample (A-1_1, A-1_2): trim_galore -q 25 –phred33 –stringency 3 –length 36 –paired A-1_1.fq.gz A-1_2.fq.gz –gzip -o ./cleandata/trim_galoredata/ … Read more
1. Quality Control of Raw Data The processing of raw data in single-cell sequencing converts the FASTQ files output from sequencers into a more analyzable format, such as a count matrix. This count matrix represents the estimated unique molecular counts for each gene in each quantified cell, sometimes categorized based on the inferred splicing status … Read more
iNature Self-assembling hydrogels for mechanical support and drug delivery have been widely studied in the context of traumatic brain injury (TBI). However, most self-assembling hydrogels rely on polymer carriers or the introduction of exogenous inactive substances, often raising biosafety concerns and making clinical translation difficult. On October 9, 2024, a research paper titled “Small Molecule … Read more