bisulfite sequencing

Bisulfite Sequencing (BSP) Technology: Principles, Processes, and Comprehensive Applications

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Bisulfite Sequencing (BSP) Technology: Principles, Processes, and Comprehensive Applications

Click the blue text above to follow us Bisulfite Sequencing (BSP) is a DNA methylation detection technology based on bisulfite conversion. The core principle is to chemically treat DNA with bisulfite, converting unmethylated cytosine (C) to uracil (U), while methylated cytosine (5-methylcytosine, 5mC) remains unchanged. The converted DNA is amplified by PCR and sequenced, allowing … Read more

Bisulfite Sequencing PCR (BSP) Method

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Bisulfite Sequencing PCR (BSP) Method

In a previous article, we discussed the prediction of CpG islands. Once a CpG island is identified, it is necessary to detect the methylation status of the corresponding region. There are various methods to detect methylation status, but the currently recognized gold standard is the bisulfite sequencing PCR (BSP). BSP Principle Genomic DNA is treated … Read more