trim-galore

Using trim-galore to remove low-quality reads and adaptersAdapters: An adapter is a known short nucleotide sequence used to link unknown target sequencing fragments.Create a new directory “trim-galore” to store the output results of trim-galore.1. If running a single sample (A-1_1, A-1_2): trim_galore -q 25 –phred33 –stringency 3 –length 36 –paired A-1_1.fq.gz A-1_2.fq.gz –gzip -o ./cleandata/trim_galoredata/ … Read more