Introduction Since 2021, Biomamba’s bioinformatics base has been producing many tutorials on single-cell sequencing, which have garnered a significant number of views. While creating these tutorials, we realized that the data from single-cell sequencing is generally very large, making it difficult for individuals to process such big data on their personal computers. Therefore, it is … Read more
1. Quality Control of Raw Data The processing of raw data in single-cell sequencing converts the FASTQ files output from sequencers into a more analyzable format, such as a count matrix. This count matrix represents the estimated unique molecular counts for each gene in each quantified cell, sometimes categorized based on the inferred splicing status … Read more
Introduction In the field of life sciences, exploring how cells undergo the entire lifecycle from birth to maturity and then to aging is crucial for understanding the development of organisms, maintaining homeostasis, and the progression of diseases. In recent years, with the advancement of single-cell sequencing technologies, scientists have been able to observe the heterogeneity … Read more