methylation

Key Points of DNA Methylation (BSP MSP) Technology

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Key Points of DNA Methylation (BSP MSP) Technology

DNA methylation is one of the earliest discovered forms of gene epigenetic modification. In eukaryotes, methylation occurs only at cytosine, where DNA methyltransferases (DNMTs) convert the 5′ end cytosine of CpG dinucleotides to 5′-methylcytosine. Numerous studies have shown that DNA methylation can induce changes in chromatin structure, DNA conformation, DNA stability, and the interaction patterns … Read more

Bisulfite Sequencing PCR (BSP) Method

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Bisulfite Sequencing PCR (BSP) Method

In a previous article, we discussed the prediction of CpG islands. Once a CpG island is identified, it is necessary to detect the methylation status of the corresponding region. There are various methods to detect methylation status, but the currently recognized gold standard is the bisulfite sequencing PCR (BSP). BSP Principle Genomic DNA is treated … Read more