DNA methylation

Gene Chip Sample Submission Secrets Column | Can Serum and Plasma cfDNA Be Used for Gene Chips?

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Gene Chip Sample Submission Secrets Column | Can Serum and Plasma cfDNA Be Used for Gene Chips?

In previous installments of the “Gene Chip Sample Submission Secrets Column,” we introduced the submission requirements for blood samples (click to read the “Gene Chip Sample Submission Secrets Column | The ‘Magical Drift’ of Blood Samples”), focusing on whole blood. So, can the components separated from whole blood be submitted? For PBMCs separated by ficoll … Read more

Bisulfite Sequencing (BSP) Technology: Principles, Processes, and Comprehensive Applications

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Bisulfite Sequencing (BSP) Technology: Principles, Processes, and Comprehensive Applications

Click the blue text above to follow us Bisulfite Sequencing (BSP) is a DNA methylation detection technology based on bisulfite conversion. The core principle is to chemically treat DNA with bisulfite, converting unmethylated cytosine (C) to uracil (U), while methylated cytosine (5-methylcytosine, 5mC) remains unchanged. The converted DNA is amplified by PCR and sequenced, allowing … Read more