Branch Linkers for ADCs: What Length is Appropriate?
Selecting different lengths of branched amino triazole linkers for site-specificMTGase-mediated antibody modification, followed by strain-promoted azide-alkyne cycloaddition (SPAAC) for payload coupling. For the payload portion, clinically validated monomethyl auristatin E (MMAE) was used, coupled with hydrophilic EGCit linkers, resulting in homogeneous ADC I (DAR 2) and ADC II/III (DAR 6).At the same time, ADC IV … Read more