Immunocytochemistry

Distinguishing Between IHC, ICC, and IF

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Distinguishing Between IHC, ICC, and IF

Immunohistochemistry, Immunocytochemistry, and Immunofluorescence are three commonly used applications in biology, with their English names being Immunohistochemistry (IHC), Immunocytochemistry (ICC), and Immunofluorescence (IF). Root meanings: Immuno- refers to immune techniques (for example, the binding of antibodies and antigens). Histo- refers to tissues (cells and their surrounding extracellular matrix). Cyto- refers to cells (excluding the extracellular … Read more

The World Under the Microscope

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The World Under the Microscope

The World Under the Microscope—— Immunocytochemistry Definition: Immunocytochemistry (ICC) is a technique established on the basis of immunology, biochemistry, and microscopy technology. It detects the presence and distribution of certain polypeptides, proteins, and large molecular substances such as membrane surface antigens and receptors in cells according to the specific binding characteristics between antigens and antibodies. … Read more

Understanding IF, ICC, and IHC Techniques

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Understanding IF, ICC, and IHC Techniques

Technical Terms are often confused with Immunofluorescence, Immunocytochemistry, and Immunohistochemistry. These three terms are often used interchangeably, which can lead to confusion when purchasing antibodies for microscopic experiments. Immunofluorescence (IF) Immunofluorescence (IF) refers to the detection method used (i.e., using fluorescent dyes to visualize the target of interest), regardless of the type of sample used. … Read more

Understanding IHC, ICC, and IF: Clearing Up Common Confusions

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Understanding IHC, ICC, and IF: Clearing Up Common Confusions

Immunohistochemistry、Immunocytochemistry and Immunofluorescence are three commonly used applications. Do you often feel confused about these three applications? They indeed have many similarities. Let’s clarify their differences. IF (Immunofluorescence) / IHC (Immunohistochemistry) / ICC (Immunocytochemistry) are three applications, and one part that everyone will definitely not confuse is “Immuno”, because all three applications involve the use … Read more