Antibody Antibodies have been analyzed by X-ray crystallography, revealing that Ig consists of four polypeptide chains connected by varying numbers of interchain disulfide bonds. Ig can form a “Y” shaped structure, known as an Ig monomer, which is the basic unit of antibodies. Basic Unit of Antibody The primary antibody specifically recognizes and binds to … Read more
BioLegend is a company focused on biomedical research, offering a wide range of products, including but not limited to the following categories: flow cytometry-related products, stem cell research products, ELISA kits, Western blotting-related products, totalseq-related products, and many immunofluorescence-related products. Immunocytochemistry (ICC) is characterized by imaging cultured primary cells or cell lines. Immunohistochemistry (IHC) refers … Read more
Technical Terms are often confused with Immunofluorescence, Immunocytochemistry, and Immunohistochemistry. These three terms are often used interchangeably, which can lead to confusion when purchasing antibodies for microscopic experiments. Immunofluorescence (IF) Immunofluorescence (IF) refers to the detection method used (i.e., using fluorescent dyes to visualize the target of interest), regardless of the type of sample used. … Read more
Immunohistochemistry、Immunocytochemistry and Immunofluorescence are three commonly used applications. Do you often feel confused about these three applications? They indeed have many similarities. Let’s clarify their differences. IF (Immunofluorescence) / IHC (Immunohistochemistry) / ICC (Immunocytochemistry) are three applications, and one part that everyone will definitely not confuse is “Immuno”, because all three applications involve the use … Read more