Abstract【Objective】To conduct a functional analysis of the myo-inositol-1-phosphate synthase (MIPS) gene GhMIPS1A, exploring its role in the development of fiber in upland cotton and its response to stress.【Methods】 The GhMIPS1A gene was studied through phylogenetic analysis, gene structure analysis, conserved motif analysis, quantitative fluorescence analysis, tobacco transient transformation, Arabidopsis overexpression tests, and virus-induced gene silencing (VIGS) technology.【Results】 The GhMIPS1A gene was cloned from upland cotton TM-1. Bioinformatics analysis revealed that the encoded protein contains four highly conserved sequences: GWGGNNG, LWTANTERY, NGSPQNTFVPGL, and SYNHLGNNDG. Subcellular localization results showed that the GhMIPS1A protein is located in the cell membrane. Overexpression of the GhMIPS1A gene in Arabidopsis significantly increased root length and more than doubled inositol content. Spatial expression pattern analysis indicated that the GhMIPS1A gene is predominantly expressed in roots, stems, leaves, and fibers, with significantly higher expression in the high fiber variety Xinluzao 18 compared to the low fiber variety Dezhimian 531. Expression pattern analysis showed that the GhMIPS1A gene has higher expression levels during the early stages of fiber development. Silencing the GhMIPS1A gene in TM-1 using VIGS technology resulted in a significant decrease in fiber density for three lines, with reductions of 4.48, 4.93, and 3.95 percentage points, and inositol content decreased by 31.83%, 32.90%, and 29.46%, respectively. Under drought or salt treatment, the expression of the GhMIPS1A gene showed a trend of first increasing and then decreasing.
【Conclusion】GhMIPS1A plays a positive role in cotton fiber development and can respond to drought and salt stress, providing genetic resources and a genetic basis for breeding high-quality, high-yield, salt-tolerant, and drought-resistant cotton varieties.
Keywords: Upland cotton; GhMIPS1A; Gene function; Fiber development; Stress resistance; Salt tolerance; Drought resistance
For reprints and forwarding, please indicate the source as follows: Cotton Journal, 2022, 34(2): 93-106.https://doi.org/10.11963/cs20210018
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