4Pi-SIM Imaging Technology for Live Cell 3D Resolution

4Pi-SIM Imaging Technology for Live Cell 3D Resolution

Subcellular organelles in cells often have intricate three-dimensional structures and biological functions. Observing the three-dimensional distribution and dynamics of organelles using fluorescence microscopy helps understand cellular functions and reveals interactions between organelles. However, fluorescence microscopy is limited by optical diffraction and the numerical aperture (NA) of the objective lens, resulting in blurred low-resolution images. Due … Read more