1. Introduction to BLASTBLAST (Basic Local Alignment Search Tool) is a bioinformatics tool provided by NCBI (National Center for Biotechnology Information) for comparing gene or protein sequences. Below are the complete steps for installing and using BLAST on a Linux system.2. Installation of BLAST01
Download the BLAST installation package
wget https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ncbi-blast-2.15.0+-x64-linux.tar.gz
02
Extract the downloaded installation package
tar -xzvf ncbi-blast-2.15.0+-x64-linux.tar.gz
03
Navigate to the bin directory inside the program folder. Enter pwd to check the current path and copy the path
cd ncbi-blast-2.15.0+/bin && pwd
04
Add environment variables; if not added, the program can be run using its absolute path
# export PATH=the copied path:$PATH, this is a temporary addition of the environment variable. You can also add “export PATH=the copied path:$PATH” to ~/.bashrc, then save and exit, and use the source ~/.bashrc command to make it effective
export PATH=”/mnt/d/soft/ncbi-blast-2.15.0+/bin:$PATH”
05
Verify if the installation was successful; if the version information is displayed correctly, the installation configuration is successful
blastn -version
