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This article focuses on the functional prediction of some common prokaryotic microbial communities in high-salinity water bodies using PICRUSt2, and the content is for reference only, please verify before use.
1. KEGG PATHWAY Level 1 Functions of Prokaryotic Microbial Communities
Environmental Information Processing
Genetic Information Processing
Organismal Systems
Cellular Processes
Human Diseases
Metabolism
2. KEGG PATHWAY Level 2 Functions of Prokaryotic Microbial Communities
Xenobiotics biodegradation and metabolism
Biosynthesis of other secondary metabolites
Metabolism of terpenoids and polyketides
Metabolism of cofactors and vitamins
Glycan biosynthesis and metabolism
Cellular community – prokaryotes
Metabolism of other amino acids
Folding, sorting and degradation
Carbohydrate metabolism
Infectious disease: bacterial
Transport and catabolism
Neurodegenerative disease
Cell growth and death
Amino acid metabolism
Environmental adaptation
Nucleotide metabolism
Replication and repair
Endocrine system
Energy metabolism
Signal transduction
Membrane transport
Lipid metabolism
Cell motility
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Translation
3. KEGG PATHWAY Level 3 Functions of Prokaryotic Microbial Communities
Biosynthesis of tropane, piperidine and pyridine alkaloids
Degradation of chlorocyclohexane and chlorobenzene
Metabolism of glycine, serine and threonine
Biosynthesis of penicillin and cephalosporin
Metabolism of phosphonates and phosphinates
Metabolism of nicotinate and nicotinamide
Synthesis and degradation of ketone bodies
Metabolism of ascorbate and aldarate
Biosynthesis of secondary bile acids
Biosynthesis of terpenoid backbone
Pathogenic cycle of Vibrio cholerae
Degradation of aminobenzoate
Metabolism of selenocompounds
Biosynthesis of streptomycin
Degradation of fluorobenzoate
Metabolism of sphingolipids
Metabolism of glycerolipids
Metabolism of vitamin B6
Metabolism of D-Alanine
Homologous recombination
Degradation of benzoate
Metabolism of pyruvate
Metabolism of riboflavin
Metabolism of tyrosine
Sulfur relay system
Methane metabolism
Toluene degradation
Purine metabolism
Protein export
4. Tips
1. Generally, bacteria are more adapted to low-salinity environments, while archaea are more adapted to high-salinity environments. Bacteria dominate in low-salinity water bodies, while archaea dominate in high-salinity water bodies.
2. The diversity and evenness of prokaryotic microbial communities often decrease with increasing salinity, possibly due to high-salinity stress inhibiting microbial diversity.
3. The primary functions of prokaryotic microbial communities in high-salinity water bodies are mainly metabolism, genetic information processing, cellular processes, and environmental information, with metabolism and genetic information processing being the most important. Additionally, carbohydrate metabolism, amino acid metabolism, metabolism of terpenoids and polyketides, metabolism of other amino acids, lipid metabolism functions, and xenobiotic biodegradation and metabolism dominate the microbial community functions.
4. The functional abundance of genetic information processing in bacterial communities increases with salinity, meaning that the higher the salinity of the water, the more intense the gene expression processes in bacterial communities. Gene expression processes include replication and repair, folding, sorting and degradation, nucleotide metabolism, and translation, which may be due to salt ion stress causing cellular damage, thereby promoting gene expression and protein translation to repair damaged cells and enhance cellular salt ion export transport capacity.
5. The functional abundance of cellular processes in archaeal communities is higher in relatively lower salinity water bodies, indicating that cellular processes in low-salinity archaeal communities are more frequent. The functional abundance of cell motility, cell growth and death, and transport and catabolism in archaeal communities decreases with increasing salinity, suggesting that archaeal communities may have a higher cell turnover rate and catabolic capacity in low-salinity environments.
6. The functional abundance of metabolism of cofactors and vitamins in archaeal communities in low-salinity environments is significantly higher than in high-salinity environments, while the functional abundance of metabolism of cofactors and vitamins in bacterial communities in high-salinity environments is significantly higher than in low-salinity environments. Vitamins and cofactors (coenzymes) usually play an important role in catalytic effects and are essential for maintaining normal metabolism. This indicates that bacteria are generally more suited to survive in low-salinity environments, while archaea are more suited to survive in high-salinity environments, suggesting that the metabolic roles of microbial cofactors and vitamins may be significantly enhanced under adverse environmental conditions.
7. Some studies indicate that chloride ions, carbonate ions, potassium ions, and magnesium ions are key influencing factors for bacterial community functions, while total dissolved solids (TDS), bicarbonate ions, carbonate ions, sodium ions, and chloride ions are key influencing factors for archaeal community functions.
