How Organoid Chips Achieve Precise Reproduction of 0%–21% Oxygen Gradients, Revealing the Mechanisms of Hypoxic Metabolic Reprogramming in Tumors and Ischemic Diseases
1. Overview The goal is to construct and stably maintain a spatial oxygen gradient ranging from normoxia (≈21% O₂ gas phase) to anoxia/zero oxygen (0% O₂ gas phase) on a reproducible and quantifiable organoid/tumor-on-chip platform, and to achieve real-time/periodic monitoring and biological sampling to elucidate hypoxia-induced metabolic reprogramming (such as HIF pathways, glycolysis/lactate production, mitochondrial … Read more