Fluorescent Subsingle Turnover Monitoring of Enzymatic Reactions Provides a Universal Platform for Directed Ultra-High-Throughput Screening
The team led by Florian Hollfelder at the University of Cambridge’s Department of Biochemistry has developed a directed ultra-high-throughput screening platform for biocatalysts in microdroplets, involving NAD(P)H enzymatic reactions and sub-single turnover detection. Enzyme engineering activities rely on functional screening to discover starting points and subsequent directed evolution. Here, ultra-high-throughput screening forms typically require optical … Read more