3D Multiparametric Photoacoustic Imaging of Tumors in Live Mice

2024.06.28. Pohang University of Science and Technology research team jointly published an article in ACS Nano (IF=15.8) titled “3D Multiparametric Photoacoustic Computed Tomography of Primary and Metastatic Tumors in Living Mice,” which describes 3D multiparametric photoacoustic computed tomography of primary and metastatic tumors in live mice.

Cover Article

Photoacoustic computed tomography (PACT) is an emerging imaging modality in preclinical cancer research that can provide multiparametric 3D information about structure, physiological function, and pharmacokinetics.

This study demonstrates the use of high-definition 3D multiparametric PACT imaging for non-invasive monitoring of primary and metastatic tumors in live mice, including monitoring of angiogenesis, carcinogenesis, hypoxia, and pharmacokinetics.

The high-definition PACT system employs a 1024-element hemispherical ultrasound transducer array, with isotropic spatial resolution of 380μm, effective field of view volume without scanning of 12.8×12.8×12.8mm^3, and acquisition time for the entire mouse body is less than 30 seconds.

First, we monitored the structural progression of the tumor microenvironment (such as angiogenesis and vascular distortion) after tumor cell inoculation. Then, we analyzed the changes in oxygen saturation in the tumor during carcinogenesis, validating the hypoxia caused in the tumor core region. Finally, after intravenous injection of micelle-encapsulated IR780 dye, we performed photoacoustic imaging of systemic pharmacokinetics and validated in vivo PACT results through fluorescence imaging both in vivo and in vitro.

By employing the PACT system and applying multiparametric analysis to subcutaneous primary tumors and metastatic liver tumors, we demonstrate that this PACT system can provide multiparametric analysis for comprehensive cancer research.

PACT System Schematic

In Vivo Structural Photoacoustic (PA) Tumor Imaging

In Vivo Functional Photoacoustic (PA) Tumor Imaging

In Vivo Contrast-Enhanced PA Imaging of Tumors

In Vivo and Ex Vivo Contrast-Enhanced Fluorescence (FL) Tumor Imaging

In Vivo Functional Photoacoustic (PA) Imaging of Metastatic TumorsEnhanced Photoacoustic (PA) Imaging of Metastatic Tumors

Article Link

https://doi.org/10.1021/acsnano.3c12551

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